BUFFERED OSMIUM TETROXIDE (OsO4) FIXATION FOR CYTOLOGICAL AND FEULGEN MICROSPECTROPHOTOMETRIC STUDIES OF HUMAN RECTAL POLYPS
نویسندگان
چکیده
Evidence has been presented that when human rectal mucosa and polyps are fixed with buffered OsO(4) according to the method of Palade, staining of paraffin sections by basic and acidic dyes results in a distinct color difference between chromatin and cytoplasm. Furthermore, the Feulgen reaction also can be carried out successfully and such OsO(4)-fixed Feulgen-stained sections are especially suitable for microspectrophotometric determinations of DNA in individual nuclei.
منابع مشابه
BUFFERED OSMIUM TETROXIDE (Os04) FIXATION FOR CYTOLOGICAL AND FEULGEN MICROSPECTROPHOTO- METRIC STUDIES OF HUMAN RECTAL POLYPS* BY CECILIE LEUCHTENBERGER, PH.D.,
The value of osmium tetroxide (OsO4) as a superior fixative for the structure of cells and tissues has been recognized for some time (1). However, staining of OsO4-fixed tissues with the usual histological stains such as hematoxylin and eosin (H and E) or with the Feulgen reaction has met with great difficulty in the past. Baker (2), who discusses OsO4 fixation in great detail, states tha t the...
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عنوان ژورنال:
- The Journal of Biophysical and Biochemical Cytology
دوره 1 شماره
صفحات -
تاریخ انتشار 1955